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To investigate the association between CT imaging traits and texture metrics with proteomic data in patients with high grade serous ovarian cancer (HGSOC).

MATERIALS AND METHODS:

This retrospective multi-institutional study enrolled 20 patients with HGSOC prior to primary cytoreductive surgery. Two readers independently assessed the contrast enhanced computed tomography (CT) and extracted 33 imaging traits. In addition all sites of suspected HGSOC were manually segmented and grey-level correlation matrix-based texture features were computed from each tumor site. Three texture features representing inter-site tumor heterogeneity were used for further analysis. Combined analysis of transcriptomics proteomics was used to identify stably expressed proteins between primary tumor sites and metastasis. The correlation between the different imaging traits and texture features with measurement of protein abundance were assessed using Kendall tau rank correlation coefficient and Mann-Whitney U test, whereby the area under the receiver operating characteristic curve (AUC) was reported as a metric of strength and direction of the association. P values < 0.05 were considered significant. 

RESULTS:

Expression of eight proteins were significantly associated with CT-based imaging traits. The strongest positive correlations was observed between peritoneal disease in the liver / right upper quadrant (P<0.001, AUC=0.940). Four proteins were associated with texture features representing inter-site tumor heterogeneity, the strongest positive correlation was between protein abundance of GSTM1 and the feature cluster dissimilarity. 

CONCLUSIONS:

This study provides first insights on potentially strong associations between standard of care CT imaging traits and CT-based texture measures of tumor burden inter-site heterogeneity and abundance of several associated proteins.

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• DICOM Image Data : 20 patient subset of TCGA-OV   Image Added
  • Click the Downloadbutton above to save a ".tcia" manifest file to your computer, which you must open with the NBIA Data Retriever.  
• Image Features:  (Summary table provided in publication)
  • 36 image features, including the 33 radiologists-scored imaging traits used in the study of Vargas et al such as lesion size and laterality, locations of peritoneal disease, nodal stations involved, and locations of metastases, and three computer-extracted texture metrics were obtained.
• Segmentations 
  • Segmentation was performed using 3DSlicer by tracing the contour of each lesion on each slice to produce the volume of interests (VOI). Voxel-wise Haralick textures (energy, entropy, contrast, and homogeneity) were computed from within the manually delineated VOIs using in-house software implemented in C++ using the Insight ToolKit. Site specific sub-regions were computed by voxel-wise clustering of the Haralick textures using kernel K-means method. Following clustering, tumor sites were divided into distinct sub-regions and summarized using average of Haralick texture measures of all voxels within that region.
• Proteomic Data
  • Protein relative abundance measurements  (link to paper and/or provide spreadsheet of data used)