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Summary


Excerpt

Pre-clinical animal models of spontaneous metastatic cancer are infrequent; the few that exist are resource intensive because determination of the presence of metastatic disease, metastatic burden, and response to therapy normally require multiple timed cohorts with animal sacrifice and extensive pathological examination. We identified and characterized a patient derived xenograft model with metastatic potential, adenocarcinoma colon xenograft 997537-175-T. In this study we performed a detailed imaging characterization (workflow below) of this model, which develops spontaneous lung metastases, details are provided in the attached standard operating procedures. Tumors in half of the mice were resected in the range 200-300 cm3 size; tumors in the other half were allowed to grow until it was necessary to euthanize them because of tumor size.

The imaging characteristics of this model (PDMR-997537-175-T), which is available from the National Cancer Institute Patient-Derived Models Repository (https://pdmr.cancer.gov/), is highly favorable for preclinical research studies of metastatic disease when used in conjunction with non-contrast T2 weighted MRI.

Results: Adenocarcinoma colon (PDMR-997537-175-T)


Table 1: Penetrance and location of pathological confirmed metastatic lesion(s).

# animals in group

# animals that displayed metastasis in MRI and confirmed by Pathology

Pathology confirmation of MRI (primary imaging site)

Other confirmed Location (s)

Mouse ID: MRI with pathology confirmation of metastasis


10 (non-resected)


6

(4 mice were EU due to xenograft size prior to observation of metastasis)


Lung


Lymph Node


1319, 1321, 1326, 1327, 1334, 1338

10 (resected)

10

Lung

Lymph Node


1320, 1323, 1324, 1325, 1328, 1329, 1332, 1333, 1335, 1337


...

PET/CT Characterization of the primary tumor:  Baseline PET (SOP attached) were performed when tumor reached an approximate 200 mm3.   Average SUVmax values (n=5) were calculated; [18F]FDG: 4.4 ± 0.6 and [18F]FLT: 1.3 ± 0.3.

Conclusion:

Excellent metastatic model with at least 60% penetrance non-resected and 100% with planned resection.  Metastases well observed on T2 MRI imaging allowing non-invasive evaluation in treatment trials.  This model can be challenging in a drug study due to the rapid xenograft tumor growth and re-growth after tumor excision.

Acknowledgements

We would like to acknowledge the individuals and institutions that have provided data for this collection:

  • Frederick National Laboratory for Cancer Research – Special Thanks to Joseph D. Kalen, PhD, Lilia V. Ileva, MS, Lisa A Riffle, Nimit Patel, Keita Saito, PhD, Yvonne Evrard, PhD, Elijah Edmondson, DVM, PhD, Jessica Phillips, Simone Difilippantonio, PhD, Chelsea Sanders, Amy James, Lia Thang, Ulrike Wagner, Yanling Liu, PhD, John B. Freymann, and Justin Kirby
  • Division of Cancer Therapeutics and Diagnosis/National Cancer Institute - James L. Tatum, MD, Paula M Jacobs, PhD, Melinda G. Hollingshead, DVM, and James H. Doroshow, MD
  • PixelMed Publishing – Special Thanks to David A. Clunie, MD
  • University of Arkansas for Medical Sciences – Special Thanks to Kirk E. Smith
  • This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261201500003I. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
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Localtab
activetrue
titleData Access

Data Access

Data TypeDownload all or Query/FilterLicense
Images (DICOM, 2.3 GB)


Tcia button generator
urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/PDMR-997537-175-T.tcia?version=1&modificationDate=1590008255458&api=v2



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labelSearch
urlhttps://nbia.cancerimagingarchive.net/nbia-search/?MinNumberOfStudiesCriteria=1&CollectionCriteria=PDMR-997537-175-T


  

(Download requires NBIA Data Retriever)

Tcia cc by 4

Standard Operating Procedure 50101 MRI T2 Weighted Non-Contrast Protocol Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated


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urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/SOP50101_MRI%20T2%20Weighted%20Non-Contrast%20Protocol_Single%20Mouse%20Pulmonary%20Gated%20and%20Multi-Mouse%20Non-Gated.pdf?version=1&modificationDate=1589916311052&api=v2



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Standard Operating Procedure 50102 Positron Emission Tomography (PET) imaging protocol


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urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/SOP50102_Positron%20Emission%20Tomography%20%28PET%29%20imaging%20protocol.pdf?version=1&modificationDate=1589916311032&api=v2



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Pdmr additional resources

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Localtab
titleDetailed Description

Detailed Description

Image Statistics

                   

Modalities

MR, SR 

Number of Subjects

24

Number of Studies

76

Number of Series

128

Number of Images

1966

Images Size (GB)1.2 GB


In addition to images, this collection includes Raw Data Storage SOP Class instances with MR Modality, generated by a Philips MR scanner; this data is not useful to anyone without the proprietary software to interpret it.


   


Localtab
titleCitations & Data Usage Policy

Citations & Data Usage Policy

Tcia limited license policy

Info
titleData Citation

Tatum, J. L., Kalen, J. D., Ileva, L. V., Riffle, L. A., Keita, S., Patel, N., Jacobs, P. M., Sanders, C., James, A., Difilippantonio, S., Thang, L., Hollingshead, M. G., Phillips, J., Evrard, Y., Clunie, D. A., Liu, Y., Suloway, C., Smith, K. E., Wagner, U., … Doroshow, J. H. (2020). Imaging characterization of a metastatic patient derived model of adenocarcinoma colon: (PDMR-997537-175-T) [Data set]. The Cancer Imaging Archive. https://doi.org/10.7937/TCIA.2020.BRY9-4N29


Info
titleTCIA Citation

Clark K, Vendt B, Smith K, Freymann J, Kirby J, Koppel P, Moore S, Phillips S, Maffitt D, Pringle M, Tarbox L, Prior F. The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository, Journal of Digital Imaging, Volume 26, Number 6, December, 2013, pp 1045-1057. DOI: https://doi.org/10.1007/s10278-013-9622-7


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Localtab
titleVersions

Version 1 (Current): Updated 2020/05/22

Data TypeDownload all or Query/Filter
Images (DICOM, 2.3 GB)


Tcia button generator
urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/PDMR-997537-175-T.tcia?version=1&modificationDate=1590008255458&api=v2



Tcia button generator
labelSearch
urlhttps://nbia.cancerimagingarchive.net/nbia-search/?MinNumberOfStudiesCriteria=1&CollectionCriteria=PDMR-997537-175-T


  

(Download requires the NBIA Data Retriever)

Standard Operating Procedure 50101 MRI T2 Weighted Non-Contrast Protocol Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated


Tcia button generator
urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/SOP50101_MRI%20T2%20Weighted%20Non-Contrast%20Protocol_Single%20Mouse%20Pulmonary%20Gated%20and%20Multi-Mouse%20Non-Gated.pdf?version=1&modificationDate=1589916311052&api=v2



Standard Operating Procedure 50102 Positron Emission Tomography (PET) imaging protocol


Tcia button generator
urlhttps://wiki.cancerimagingarchive.net/download/attachments/70225394/SOP50102_Positron%20Emission%20Tomography%20%28PET%29%20imaging%20protocol.pdf?version=1&modificationDate=1589916311032&api=v2



Pdmr additional resources





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