Summary


Pre-clinical animal models of spontaneous metastatic cancer are infrequent; the few that exist are resource intensive because determination of the presence of metastatic disease, metastatic burden, and response to therapy normally require multiple timed cohorts with animal sacrifice and extensive pathological examination. We identified and characterized a patient derived xenograft model with metastatic potential, adenocarcinoma pancreas xenograft 292921-168-R. In this study we performed a detailed imaging characterization (workflow below) of this model, which develops spontaneous lung metastases, details are provided in the attached standard operating procedures. Tumors in half of the mice were resected in the range 200-300 cm3 size; tumors in the other half were allowed to grow until it was necessary to euthanize them because of tumor size.



The imaging characteristics of this model (PDMR-292921-168-R), which is available from the National Cancer Institute Patient-Derived Models Repository (https://pdmr.cancer.gov/), is highly favorable for preclinical research studies of metastatic disease when used in conjunction with non-contrast T2 weighted MRI.

Results: Adenocarcinoma pancreas (PDMR-292921-168-R)


Table 1: Penetrance and location of pathological confirmed metastatic lesion(s).

# animals in Group

# animals that displayed metastasis in MRI and confirmed by Pathology

Pathology confirmation of MRI (primary imaging site)

Other confirmed Location (s)

Mouse ID: MRI with pathology confirmation of metastasis

10 (non-resected)

4   

(5 mice were EU due to xenograft size prior to observation of metastases)

Lung

Lung

1409, 1410, 1418, 1423

10 (resected)

10

Lung

Lung, Kidney, Nodes, Peritoneal Wall

1405, 1407, 1408, 1411, 1413,1415, 1416, 1419, 1420, 1422




Percent penetrance with respect to the average time-to-metastasis for non-resected (plot A: time from implant) and resected (plot B: time from tumor resection) cohorts.


Plot A

Plot B


PET/CT Characterization of the primary tumor:  Baseline PET (SOP available below) were performed when tumor reached an approximate 200 mm3.   Average SUVmax values (n=5) were calculated; [18F]FDG: 1.3 ± 0.2 and [18F]FLT: 2.3 ± 0.6.

Conclusion

Excellent metastatic model with at least 50% penetrance un-resected and 100% with planned early resection.  Metastases are well observed on T2 MRI imaging allowing non-invasive evaluation in treatment trials.

Acknowledgements

We would like to acknowledge the individuals and institutions that have provided data for this collection:



Data Access

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Data TypeDownload all or Query/Filter
Images (DICOM, 1.6 GB)

 

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PDMR Patient Specimen (external)

Standard Operating Procedure 50101: MRI T2 Weighted Non-Contrast Protocol: Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated

Standard Operating Procedure 50102: Positron Emission Tomography (PET) imaging protocol


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Detailed Description


Image Statistics


Modalities

MR, SR

Number of Subjects

20

Number of Studies

89

Number of Series

160

Number of Images

2657

Images Size (GB)1.6 GB






Citations & Data Usage Policy

Tatum, J., Kalen, J., Ileva, lilia, L, R., S, K., N, P., Jacobs, P., Sanders, C., A, J., Difilippantonio, S., L, T., hollingshead, melinda, J, P., Y, E., Clunie, D., Y, L., Suloway, C., Smith, K., U, W., … Doroshow, J. (n.d.). Imaging characterization of a metastatic patient derived model of adenocarcinoma pancreas: PDMR-292921-168-R. The Cancer Imaging Archive. https://doi.org/10.7937/TCIA.2020.PCAK-8Z10




This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261201500003I. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.




Clark K, Vendt B, Smith K, Freymann J, Kirby J, Koppel P, Moore S, Phillips S, Maffitt D, Pringle M, Tarbox L, Prior F. The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository, Journal of Digital Imaging, Volume 26, Number 6, December, 2013, pp 1045-1057. DOI: 10.1007/s10278-013-9622-7


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Version 1 (Current): Updated 2020/03/23


Data TypeDownload all or Query/Filter
Images (DICOM, 1.6 GB)

(Requires NBIA Data Retriever .)

PDMR Patient Specimen (external)

SOP50101: MRI T2 Weighted Non-Contrast Protocol: Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated