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Pre-clinical animal models of spontaneous metastatic cancer are infrequent; the few that exist are resource intensive because determination of the presence of metastatic disease, metastatic burden, and response to therapy normally require multiple timed cohorts with animal sacrifice and extensive pathological examination. We identified and characterized a patient derived xenograft model with metastatic potential, adenocarcinoma pancreas xenograft 521955-158-R4. In this study we performed a detailed imaging characterization (workflow below) of this model, which develops spontaneous lung metastases, details are provided in the attached standard operating procedures. Tumors in half of the mice were resected in the range 200-300 mm3 size; tumors in the other half were allowed to grow until it was necessary to euthanize them because of tumor size.

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The imaging characteristics of this model (PDMR-521955-158-R4), which is available from the National Cancer Institute Patient-Derived Models Repository (https://pdmr.cancer.gov/), is highly favorable for preclinical research studies of metastatic disease when used in conjunction with non-contrast T2 weighted MRI.

Results: Adenocarcinoma pancreas (PDMR-521955-158-R4)

Table 1: Penetrance and location of pathological confirmed metastatic lesion(s).

# animals in Group

# animals that displayed metastasis in MRI and confirmed by Pathology

Pathology confirmation of MRI (primary imaging site)

Other confirmed Location (s)

Mouse ID: MRI with pathology confirmation of metastasis


10 (non-resected)

4

(5 mice were EU due to xenograft size prior to observation of metastases)

Lung

Lung

2163, 2169, 2171, 2179


10 (resected)

7

Lung

Lung (70%)
Para-vertebral muscle (50%)
Nodal disease (10%)

2160, 2161, 2164, 2170, 2172, 2176, 2178


Percent penetrance with respect to the average time-to-metastasis for non-resected (time from implant: 10.7 ± 1.8 weeks) and resected (time from tumor resection: 5.3 ± 1.1 weeks) cohorts.


PET/CT Characterization of the primary tumor:  Baseline PET (SOP attached) were performed when tumor reached an approximate 200 mm3.   Average SUVmax values (n=6) were calculated; [18F]FDG: 2.3 ± 0.8 and [18F]FLT: 2.4 ± 0.4.

Conclusion:

Good metastatic model with 40% penetrance un-resected and 70% with planned early resection.  Metastases are well observed on T2 MRI imaging allowing non-invasive evaluation in treatment trials.


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titleData Access

Data Access

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Standard Operating Procedure 50101: MRI T2 Weighted Non-Contrast Protocol:  Single Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated (pdf, 141 kB)


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SOP50101_MRI T2 Weighted Non-Contrast Protocol _ Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated.pdf


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Standard Operating Procedure 50102: Positron Emission Tomography (PET) imaging protocol (pdf, 165 kB)



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Pdmr additional resources

The NCI Cancer Research Data Commons (CRDC) provides access to additional data and a cloud-based data science infrastructure that connects data sets with analytics tools to allow users to share, integrate, analyze, and visualize cancer research data.


Localtab
titleDetailed Description

Detailed Description

Image Statistics

Radiology Image Statistics

Modalities

MR,SR

Number of Patients

20

Number of Studies

142

Number of Series

264

Number of Images

4608

Images Size (GB)2.8




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titleCitations & Data Usage Policy

Citations & Data Usage Policy

Tcia limited license policy

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titleData Citation

Tatum, J., Kalen, J., Jacobs, P., Ileva, lilia, Riffle, L., Keita, S., Patel, N., Sanders, C., James, A., Difilippantonio, S., Thang, L., Hollingshead, M., Evrard, Y., Edmondson, E., Clunie, D., Liu, Y., Suloway, C., Smith, K., Wagner, U., Freymann, J. B., Kirby, J., Doroshow, J. (2022).Imaging characterization of a metastatic patient derived model of adenocarcinoma pancreas: (PDMR-521955-158-R4) (Version 1) [Data set]. The Cancer Imaging Archive. https://doi.org/10.7937/q37d-vh79 


Info
titleTCIA Citation

Clark K, Vendt B, Smith K, Freymann J, Kirby J, Koppel P, Moore S, Phillips S, Maffitt D, Pringle M, Tarbox L, Prior F. The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository, Journal of Digital Imaging, Volume 26, Number 6, December, 2013, pp 1045-1057. DOI: 10.1007/s10278-013-9622-7

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titleVersions

Version 1 (Current): Updated 2022/08/17

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labelSearch


(Download requires the NBIA Data Retriever)

Standard Operating Procedure 50101: MRI T2 Weighted Non-Contrast Protocol: Single Mouse Pulmonary Gated and Multi-Mouse Non-Gated


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urlhttps://wiki.cancerimagingarchive.net/download/attachments/127665355/SOP50101_MRI%20T2%20Weighted%20Non-Contrast%20Protocol_Single%20Mouse%20Pulmonary%20Gated%20and%20Multi-Mouse%20Non-Gated.pdf?api=v2



Standard Operating Procedure 50102: Positron Emission Tomography (PET) imaging protocol






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