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  • Comparison of mIF versus mIHC for immune markers in head and neck carcinoma (HNSCC-mIF-mIHC-comparison)

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With advancement of immunotherapies, there has been a paradigm shift in the standard of care for cancer treatment and in the focus of cancer research leveraging the immune system in the tumor immune microenvironment. Therefore, accurate characterization of the tumor microenvironment in each disease site is extremely important. We compared multiplex immunofluorescence assay (mIF) using multispectral microscopy and multiplex immunohistochemistry assay (mIHC). Here we report the comparison of these two assays regarding data acquisition, image analyses, and concordance in marker intensities and characterization of the microenvironmnent. microenvironment.  This is the first direct comparison of mIF and mIHC using the identical slides. It provides a standardized dataset to demonstrate the equivalence of the two methods and a source that can be used to calibrate other methods.  Images were taken at 20x magnification.

Acknowledgments

This work has been supported by the James and Esther King Biomedical Research Grant (7JK02) and Moffitt Merit Society Award to C. H. Chung. It is also supported in part by the Moffitt’s Total Cancer Care Initiative, Collaborative Data Services, Biostatistics and Bioinformatics, and Tissue Core Facilities at the H. Lee Moffitt Cancer Center & Research Institute, an NCI-designated Comprehensive Cancer Center (P30-CA076292).


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activetrue
titleData Access

Data Access

Data TypeDownload all or Query/Filter
Tissue Slide Images (TIFF, IM3, 8.96 GB)

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urlhttps://pathology.cancerimagingarchive.net/pathdata/select.html?filter=%7B%22study%22%3A%7B%22match%22%3A%22mIF_vs_mIHC%22%7D%2C%22subject_id%22%3A%7B%7D%7D

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titleDetailed Description

Detailed Description

Image Statistics


Modalities

Pathology

Number of Subjects

8

Number of Images

864

Images Size (GB)8.96

Images were taken at 20x magnification.



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titleCitations & Data Usage Policy

Citations & Data Usage Policy

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titleData Citation

Slebos, R. J. C., de la Iglesia, J. V., Aden-Buie, G. A., Chaudhary, R., Hernandez-Prera, J. C., Chung, C. H., & Gerke, T. A. (2020). Comparison of mIF versus mIHC for immune markers in head and neck carcinoma [Data set]. The Cancer Imaging Archive. https://doi.org/10.7937/TCIA.2020.T90F-WB82


Info
titleAcknowledgement

This work has been supported by the James and Esther King Biomedical Research Grant (7JK02) and Moffitt Merit Society Award to C. H. Chung. It is also supported in part by the Moffitt’s Total Cancer Care Initiative, Collaborative Data Services, Biostatistics and Bioinformatics, and Tissue Core Facilities at the H. Lee Moffitt Cancer Center & Research Institute, an NCI-designated Comprehensive Cancer Center (P30-CA076292).


Info
titleTCIA Citation

Clark K, Vendt B, Smith K, Freymann J, Kirby J, Koppel P, Moore S, Phillips S, Maffitt D, Pringle M, Tarbox L, Prior F. The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository, Journal of Digital Imaging, Volume 26, Number 6, December, 2013, pp 1045-1057. DOI: 10.1007/s10278-013-9622-7

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Localtab
titleVersions

Version 1 (Current): Updated 2020/06/04

Data TypeDownload all or Query/Filter
Tissue Slide Images (TIFF, IM3, 8.96 GB)

Tcia button generator
urlhttps://app.box.com/s/norlxmond7s7nwtexkb9a1arpdsss2ww
Tcia button generator
labelSearch
urlhttps://pathology.cancerimagingarchive.net/pathdata/select.html?filter=%7B%22study%22%3A%7B%22match%22%3A%22mIF_vs_mIHC%22%7D%2C%22subject_id%22%3A%7B%7D%7D



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