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  • Comparison of mIF versus mIHC for immune markers in head and neck carcinoma (HNSCC-mIF-mIHC-comparison)
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Summary

With advancement of immunotherapies, there has been a paradigm shift in the standard of care for cancer treatment and in the focus of cancer research leveraging the immune system in the tumor immune microenvironment. Therefore, accurate characterization of the tumor microenvironment in each disease site is extremely important. We compared multiplex immunofluorescence assay (mIF) using multispectral microscopy and multiplex immunohistochemistry assay (mIHC). Here we report the comparison of these two assays regarding data acquisition, image analyses, and concordance in marker intensities and characterization of the microenvironment.  This is the first direct comparison of mIF and mIHC using the identical slides. It provides a standardized dataset to demonstrate the equivalence of the two methods and a source that can be used to calibrate other methods.  Images were taken at 20x magnification.

Acknowledgments

This work has been supported by the James and Esther King Biomedical Research Grant (7JK02) and Moffitt Merit Society Award to C. H. Chung. It is also supported in part by the Moffitt’s Total Cancer Care Initiative, Collaborative Data Services, Biostatistics and Bioinformatics, and Tissue Core Facilities at the H. Lee Moffitt Cancer Center & Research Institute, an NCI-designated Comprehensive Cancer Center (P30-CA076292).


Data Access

Data TypeDownload all or Query/Filter
Tissue Slide Images (TIFF, IM3, 8.96 GB)

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Detailed Description

Image Statistics


Modalities

Pathology

Number of Subjects

8

Number of Images

864

Images Size (GB)8.96

Citations & Data Usage Policy

Users of this data must abide by the Creative Commons Attribution 4.0 International License under which it has been published. Attribution should include references to the following citations:

Data Citation

Slebos, R. J. C., de la Iglesia, J. V., Aden-Buie, G. A., Chaudhary, R., Hernandez-Prera, J. C., Chung, C. H., & Gerke, T. A. (2020). Comparison of mIF versus mIHC for immune markers in head and neck carcinoma [Data set]. The Cancer Imaging Archive. https://doi.org/10.7937/TCIA.2020.T90F-WB82

Acknowledgement

This work has been supported by the James and Esther King Biomedical Research Grant (7JK02) and Moffitt Merit Society Award to C. H. Chung. It is also supported in part by the Moffitt’s Total Cancer Care Initiative, Collaborative Data Services, Biostatistics and Bioinformatics, and Tissue Core Facilities at the H. Lee Moffitt Cancer Center & Research Institute, an NCI-designated Comprehensive Cancer Center (P30-CA076292).

TCIA Citation

Clark K, Vendt B, Smith K, Freymann J, Kirby J, Koppel P, Moore S, Phillips S, Maffitt D, Pringle M, Tarbox L, Prior F. The Cancer Imaging Archive (TCIA): Maintaining and Operating a Public Information Repository, Journal of Digital Imaging, Volume 26, Number 6, December, 2013, pp 1045-1057. DOI: 10.1007/s10278-013-9622-7

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Version 1 (Current): Updated 2020/06/04

Data TypeDownload all or Query/Filter
Tissue Slide Images (TIFF, IM3, 8.96 GB)



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